site stats

Pherd30t

Web19. aug 2024 · technology into the pHERD30T vector amplified with primers pHERD30T_forward and pHERD30T_reverse (table S1). Clinical strains were transformed … Web13. apr 2024 · 将pherd30t及重组质粒pherd30t-ypjd转化入营养缺陷菌株e.coliwm3064，并通过接合的方式进入宿主工程菌株pv-4δypjd中，空载pherd30t转化入营养缺陷菌 …

pCas3cRh载体图谱质粒图谱、序列、价格、抗性、测序引物、大 …

Web19. nov 2015 · KD604 cells carrying pCas and pCsy expressing wild-type cas/csy genes were transformed with compatible pHERD30T-based plasmids expressing various anti-CRISPR proteins (AcrFs) listed on the right (acrF1-fs carries a frame-shift mutation). Expression of plasmid-borne gene was induced and spacer acquisition was monitored by PCR … WebThe recombinant pHERD30T_GFP(ASV) vector. The recombinant pHERD30T vector, encoding the GFP(ASV) protein was obtained by cloning the gfp(ASV) gene into the … is dragonfire mod free https://ashleywebbyoga.com

Comparison of three Agrobacterium-mediated co-transformation …

WebpHERD30T-mucE BioVector NTCC质粒载体菌株细胞基因保藏中心. 价 格： ¥98755. 货 号： pHERD30T-mucE. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！. Web23. jan 2015 · 噬菌体内溶素基因分离、鉴定及功能研究.pdf. 噬菌体内溶素基因分离、鉴定及功能研究,噬菌体,t4噬菌体,噬菌体展示,t2噬菌体,噬菌体展示技术,温和噬菌体,烈性噬菌体, … Webtechnology into the pHERD30T vector amplified with primers pHERD30T_forward and pHERD30T_reverse (table S1). Clinical strains were transformed as previously described [18]. Redox sensitivity assay Strains were grown o. n. in 50% LB medium containing 0% or 2% MIC of azithromycin. Cultures were then mixed with molten 0.5% ryan field wabc

(PDF) Characterization of the self-targeting Type IV CRISPR ...

Category:CRISPR-Cas12a targeting of ssDNA plays no detectable role in …

Tags:Pherd30t

Pherd30t

随着致病性细菌耐药性的加剧寻找有效且不易造成菌株耐药性的抗 …

WebCharacterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans Web7. jún 2024 · Each donor strain carried unmodified pHERD30T or a derivative encoding the complementary protospacer sequence on the leading strand (LDS) or lagging strand (LGS) with or without a PAM (Supplementary Figure S3). The strains were grown overnight in LB broth supplemented with the appropriate antibiotics and CRISPR-Cas inducers. Donor and …

Pherd30t

Did you know?

http://www.biofeng.com/zaiti/dachang/pRSFDuet-1.html Web10. okt 2008 · RNA was extracted from log-phase B. pseudomallei Bp50 cells harboring pHERD30T that either had no arabinose added (None) or were induced for 2 h by the addition of the indicated amounts of l-arabinose. Quantitative real-time PCR was performed by using lacZα-specific primers. Data were normalized by using the 23S rRNA gene as the …

Web19. nov 2014 · 将rie、e、e1 87、e34 157和e110 157基因克隆到pherd30t载体上 通过生长曲线的测定 初步确定e蛋白和改造后的rie蛋白、e1 87蛋白、e34 157蛋白和e110 157蛋白对 … Web1. jan 2024 · Transform 50 ng of pHERD30T and pHERD30T-targ in commercially available chemically competent E. coli DH5α (subcloning efficiency) as per manufacturer's instructions.-Add 950 µL of LB to the transformation mix, incubate at 37 ˚C for 1h at 180 rpm.-Plate 50 µL on LB agar supplemented with 30 µg/mL of gentamicin sulfate-

WebWe describe here the use of pHERD30T plasmid, a P BAD -based shuttle vector carrying a gentamicin resistance marker [9]. This plasmid was selected because it is a well … Web7. jún 2024 · PAO1 tn7::LbCas12a pHERD30T-crRNA-RFP was used as the non-targeting control and PAO1 tn7::LbCas12a pHERD30T-crRNA-DMS3 was used for targeting. …

Web27. máj 2024 · The resulting pHERD30T plasmids were transferred to P. aeruginosa strains by either chemical transformation or tripartite conjugation using the pRK600 plasmid as …

WebEscherichia-Pseudomonas shuttle vector pHERD30T: eukaryotic vectors: Activation tagging vector pGA2772: Activation tagging vector pSKI074: Adenoviral expression vector Ad … ryan fieldhouse northwestern costhttp://www.biovector.net/product/2326461.html ryan fields wustlWeb5. jún 2024 · We were successful in removing selectable marker genes in transgenic B. napus plants using all three co-transformation systems developed in this study. It was … is dragonflight alt friendlyWeb5. feb 2024 · The recombinant pHERD30T_GFP(ASV) vector. The recombinant pHERD30T vector, encoding the GFP(ASV) protein was obtained by cloning the gfp(ASV) gene into … is dragonfire ward worth itWebpCas3cRh. 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务： 质粒构建 基因合成 质粒大提. 立刻购买. ryan fightmaster mdWeb10. mar 2024 · sites of pHERD30T. For experiments in which plasmid-borne crRNAs were used, the resulting plasmids were introduced into PAO1 tn7::LbCas12a directly via electroporation and selection on LB supplemented with 50 μg/ml gentamicin. To integrate crRNAs into the chromosome, the region encompassing the araC ryan fimmen ohio state universityWeb10. mar 2024 · sites of pHERD30T. For experiments in which plasmid-borne crRNAs were used, the resulting plasmids were introduced into PAO1 tn7::LbCas12a directly via … ryan fierst