Web23 mei 2013 · TROUBLESHOOTING. TROUBLESHOOTING. Our ... Steps 5–13, ORO staining: 2 h in total. Steps 14–16, acquisition and quantification of images: 2–6 h, depending on the number of subjects and tissues. WebIncorrect flow rate. Ensure that your samples are being run at the lowest flow rate setting on your cytometer. High flow rates will give rise to high coefficients of variation (CVs), leading to a loss of resolution of the different phases of the cell cycle. Insufficient staining with Propidium Iodide/RNase (PI) solution.
H&E Staining in Microscopy Science Lab Leica Microsystems
WebUse EM-grade glutaraldehyde freshly diluted from ampules. Choose longer wavelength channels for low-abundance targets. Use normal serum from the same species as the secondary antibody used. Consider a charge-based blocker, such as Image-iT® FX Signal Enhancer #11932, depending on the source of background. WebWhen staining blood and bone marrow smears, the pH of the staining solution and/or buffer is a critical factor. Technical Procedure Immersion Staining Protocol 1. Thoroughly dry blood or bone marrow smears. 2. Fix smears in absolute methanol for 15 seconds to 5 minutes 3. Stain smears in Wright-Giemsa Stain Solution for 1 minute. 4. rechute traduction
Troubleshooting in the histology laboratory: Journal of …
Web12 feb. 2014 · Troubleshooting in H&E Staining Manan Shah 25k views • 29 slides Gross Examination, Selection, Collection and Fixation of Specimen ghulam abbas 19.7k views • 25 slides 16 histotechniques 2 Nepalese army institute of health sciences 24.2k views • 34 slides More Related Content Slideshows for you (20) Tissue Processing in Histopathology WebEcoDye™ DNA Staining Solution 10 ㎕ 0.5X TAE buffer agarose solution 100 ㎖/ EcoDye™ DNA Staining Solution 10 ㎕ 1.0X TAE buffer Detection using UV imaging Rice multiplex product 1: 12㎕2: 10㎕3: 8㎕ 4: 6 ㎕5: 4 6: 2 Trouble shooting guide 솔젠트㈜제품사용시우선Check 사항 WebAdd the primary antibody and incubate at 4°C overnight with gentle agitation. Wash (3 x 15 min) in 0.1M PBS/0.3% Triton. Add secondary antibody either for 2 h at room temperature or overnight at 4°C with gentle agitation. Wash (3 x 15 min) in 0.1M PBS (no Triton). Wash once briefly in 0.1M acetate buffer to remove PBS. rechversv formblatt