WebClickSeq is an RNA sequencing technique that uses bioconjugation as an alternative to fragmentation in the library preparation step, to produce lower error rates than standard … WebAug 14, 2015 · ClickSeq is random-primed and can sequence any (unfragmented) RNA template, while PAC-seq is targeted to poly(A) tails of mRNAs. Here, we further develop …
Development of Poly(A)-ClickSeq as a tool enabling simultaneous …
WebClickSeq illustrates how robust, bio-orthogonal chemistry can be harnessed in vitro to capture and dissect complex biological processes. Here, we describe an updated protocol for the synthesis of “ClickSeq” libraries. Original language: English (US) Title of host publication: Methods in Molecular Biology: Publisher: WebAug 14, 2015 · Importantly, ClickSeq is robust against common artifacts of NGS such as chimera formation and artifactual recombination with fewer than 3 aberrant events … deba zarautz oinez
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WebClickSeq illustrates how robust, bio-orthogonal chemistry can be harnessed in vitro to capture and dissect complex biological processes. Here, we describe an updated protocol for the synthesis of “ClickSeq” libraries. less. Related articles. Based on techniques. WebMar 10, 2024 · genetic variants, we have combined ‘ClickSeq’ with tiled-amplicon approaches. ClickSeq (22, 23) is a click-chemistry-based platform for NGS that prevents artifactual sequence chimeras in the output data (24). Using ClickSeq, the 3’end of an amplified cDNA segment is generated by available under aCC-BY-ND 4.0 International … WebMar 11, 2024 · Tiled-ClickSeq therefore provides a convenient and robust platform for SARS-CoV-2 genomics that captures the full range of RNA species in a single, simple assay. ### Competing Interest Statement E.J. and A.R. are co-founders and owners of ClickSeq Technologies LLC, a Texas-based Next-Generation Sequencing provider … deba projektbau gmbh