WebFeb 4, 2024 · CountBright Absolute Counting Beads: ThermoFisher Scientific: Cat# C36950: BD Quantibrite Beads: BD Biosciences: Cat# 340495: Lead Contact and Materials Availability. Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Scott M. Krummey ([email protected]). This … WebOrder number : C36950 (Ex-work price) $ On demand. Add to Wishlist. Qty: add to cart. Kits & Chemicals ... CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). ...
CountBright™ 绝对计数微珠,用于流式细胞分析
WebJun 20, 2014 · LSR II cell counting requires counting beads because we cannot know with precision the exact sample volume the instrument is aspirating. Example Experiment: Human peripheral blood mononuclear cells ... 50 µl of Invitrogen CountBright beads (catalog # C36950) was added to the sample. Vortex the beads vigorously before adding them to … projection comparator
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Web• CountBright Absolute counting beads (Invitrogen, sold by ThermoFisher Scientifc #C36950) • OneComp eBeads Compensation Beads (Invitrogen, sold by ThermoFisher Scientifc #01-1111-41) • Fluorophore-conjugated antibodies (see Tables 1-3); eBioscience antibodies are ordered through ThermoFisher Scientific PROTOCOL WebParticle counting kit if working in particles/mL (Invitrogen Cat. no. B7277, C36950) Whole blood sample collected in sodium heparin collection tube Water bath or incubator set to 37ºC Ice bath/bucket Analysis tubes for your flow cytometer Water bath sonicator Centrifuge Flow cytometer with 488 nm excitation wavelength (argon-ion laser ... WebCounting Beads and these “Pre-Column” samples are analyzed on a flow cytometer to capture Cell:Bead ratios. The remaining portion (majority) of the blood sample is applied to a Miltenyi LS Column ... CountBrightTM Absolute Counting Beads (cat # C36950, Invitrogen) 5. Anti-Rat CD59-PE Antibody (MutaFlowPLUS kit) and Anti-Rodent CD61-PE projection countdown timer